Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

نویسندگان

  • Kristyna Hrncirova
  • Martina Lengerova
  • Iva Kocmanova
  • Zdenek Racil
  • Pavlina Volfova
  • Dita Palousova
  • Mojmir Moulis
  • Barbora Weinbergerova
  • Jana Winterova
  • Martina Toskova
  • Sarka Pospisilova
  • Jiri Mayer
چکیده

We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 48 9  شماره 

صفحات  -

تاریخ انتشار 2010